Warner Laboratory: Murine Model for Small Bowel Resection

The mouse, because of its small size, typically has presented a formidable technical challenge for safe resection and reanastomosis of the small bowel. With the advent of transgenic technology, the mouse is the ideal animal for characterization of adaptation following small bowel resection, because the expression of candidate factors considered essential may be genetically abolished or amplified. Further, transgenic models permit a means to understand temporal, spatial and stimulus-responsive regulation of gene expression. Our laboratory has perfected a murine model for small bowel resection with a resultant survival of greater than 80 percent.

Related Publications
Tantemsapya N, Meinzner-Derr J, Erwin CR, Warner BW: Body composition and metabolic changes associated with massive intestinal resection in mice. J Pediatr Surg 43:14-19, 2008.

Nair R, Warner BB, Warner BW: Role of EGF and other growth factors in the prevention of necrotizing enterocolitis. Semin Perinatol 32: 107-113, 2008.

Martin CA, Bernabe KQ, Taylor JA, Nair R, Paul RJ, Guo J, Erwin CR, Warner BW: Resection-induced intestinal adaptation and the role of enteric smooth muscle. J Pediatr Surg 43:1011-1017, 2008.

Taylor JA, Martin CA, Nair R, Guo J, Erwin CR, Warner BW: Lessons learned: optimization of the murine small bowel resection model. J Pediatr Surg 43(6):1018-1024, 2008.

Guo J, Longshore S, Nair R, Warner BW: pRb, but not p107 or p130 is required for maintenance of enterocyte quiescence and differentiation in small intestine. J Biol Chem 284: 134-140, 2009.

Longshore SW, Wakeman D, McMellen M, Warner BW: Bowel resection induced intestinal adaptation: Progress from bench to bedside. Minerva Pediatrica 61: 239-251, 2009.

Longshore SW, Nair R, Perrone EE, Erwin CR, Guo J, Warner BW: p21waf1/cip1 deficiency does not perturb the intestinal crypt stem cell population after massive small bowel resection. J Pediatr Surg 44: 1065-1071, 2009.

Martin CA, Perrone EE, Longshore SW, Toste P, Bitter K, Nair R, Guo J, Erwin CR, Warner BW: Intestinal resection induces angiogenesis within adapting intestinal villi. J Pediatr Surg 44: 1077-1083, 2009.

Qiu Z, Longshore SW, Warner BW, Rudnick DA: Murine functional liver mass is reduced following partial small bowel resection. J Gastrointest Surg 13: 2176-2182, 2009.

McMellen ME, Wakeman D, Longshore SW, McDuffie L, Warner BW: Growth factors: possible roles for clinical management of the short bowel syndrome. Semin Pediatr Surg 19: 35-43, 2010

Najaf TA, Vachharajani NA, Warner BW, Vachharajani AJ: Interval between clinical presentation of necrotizing enterocolitis and bowel perforation in neonates. Pediatr Surg Int, 26: 607-609, 2010.

Perrone EE, Chen C, Longshore SW, Okezie O, Warner BW, Sun CC, Alaish SM, Strauch ED: Dietary bile acid supplementation improves intestinal integrity and survival in a murine model. J Pediatr Surg, 45: 1256-1265, 2010.

Wakeman D, Longshore SW, McMellen ME, Santos JA, Guo J, Erwin CR, Warner BW: Extent of small bowel resection does not influence the magnitude of intestinal adaptation in the mouse. J Pediatr Surg 45: 1274-1279, 2010.

McMellen ME, Wakeman D, Erwin CR, Guo J, Warner BW: Epidermal growth factor receptor signaling modulates chemokine (CXC) ligand 5 expression and is associated with villus angiogenesis following small bowel resection. Surgery 148(2):364-70, 2010.

Bucher BT, Guth RM, Saito JM, Najaf T, Warner BW: Impact of hospital volume on in-hospital mortality of infants undergoing repair of congenital diaphragmatic hernia. Ann Surg. 252(4):635-42, 2010.

Bucher BT, Guth RM, Elward AM, Hamilton NA, Dillon PA, Warner BW, Keller MS: Risk factors and outcomes of surgical site infection in children. J Am Coll Surg. 212(6): 1033-1038, 2011.

Bucher BT, Warner BW, Dillon PA: Antibiotic prophylaxis and the prevention of surgical site infection. Curr Opin Pediatr. 23(3):334-8, 2011.

Bucher BT, McDuffie LA, Shaikh N, Tarr PI, Warner BB, Hamvas A, White FV, Erwin CR, Warner BW: Bacterial DNA content in the intestinal wall from infants with necrotizing enterocolitis. J Pediatr Surg. 46(6):1029-33, 2011.

McDuffie LA, Bucher BT, Erwin CR, Wakeman D, White FV, Warner BW: Intestinal adaptation after small bowel resection in human infants. J Pediatr Surg. 46(6):1045-51, 2011.

Bucher BT, Hall BL, Warner BW, Keller MS: Intussusception in children: cost-effectiveness of ultrasound vs diagnostic contrast enema. J Pediatr Surg.  46(6):1099-105, 2011.

Lawal TA, Falcone RA, von Allmen D, Denson LA, Levitt MA, Warner BW, Frischer JS: The utility of routine pouchogram before ileostomy reversal in children and adolescents following ileal pouch anal anastomosis. J Pediatr Surg. 46(6):1222-5, 2011.

Leinicke JA, Longshore S, Wakeman D, Guo J, Warner BW: Regulation of Retinoblastoma Protein (Rb) by p21 Is Critical for Adaptation to Massive Small Bowel Resection. J Gastrointest Surg. 2011 Nov 1. [Epub ahead of print]

Yao J, Maslov KI, Puckett ER, Rowland KJ, Warner BW, Wang LV: Double-illumination photoacoustic microscopy. Opt Lett 37:659-61, 2012.

Wakeman D, Guo J, Santos JA, Wandu WS, Schneider JE, McMellen ME, Leinicke JA, Erwin CR, Warner BW: p38 MAPK regulates Bax activity and apoptosis in enterocytes at baseline and after intestinal resection. Am J Physiol Gastrointest Liver Physiol, 302: G997-G1005, 2012.

Hitch MC, Leinicke JA, Wakeman D, Guo J, Erwin CR, Rowland KJ, Merrick EC, Heuckeroth RO, Warner BW: Ret heterozygous mice have enhanced intestinal adaptation after massive small bowel resection. Am J Physiol Gastrointest Liver Physiol, 302: G1143-G1150, 2012.

Rowland KJ, Yao J, Wang L, Erwin CR, Maslov KI, Wang LV, Warner BW: Immediate alterations in intestinal oxygen saturation and blood flow following massive small bowel resection as measured by photoacoustic microscopy. J Pediatr Surg 47: 1143-1149, 2012.

Wakeman D, Schneider JE, Liu J, Wandu WS, Erwin CR, Guo J, Stappenbeck TS, Warner BW: Deletion of p38-alpha MAPK within enterocytes promotes colon tumorigenesis. Surgery 152: 286-293, 2012.

Rowland KJ, McMellen ME, Erwin CR, Wandu WS, Warner BW: Enterocyte expression of epidermal growth factor receptor is not required for intestinal adaptation in response to massive small bowel resection. J Pediatr Surg 47: 1748-1753, 2012.

Yu L, Wynn J, Ma L, Guha S, Mychaliska GB, Crombleholme TM, Azarow KS, Lim FY, Chung DH, Potoka D, Warner BW, Bucher B, LeDuc CA, Costa K, Stolar C, Aspelund G, Arkovitz M, Chung WK: De novo copy number variants are associated with congenital diaphragmatic hernia. J Med Genet 49: 650-659, 2012.